ITC Isothermal Titration Calorimetry
Isothermal Titration Calorimetry (ITC) is a technique used in quantitative studies of a wide variety of biomolecular interactions. It works by directly measuring the heat that is either released or absorbed during a biomolecular binding event.
ITC is the only technique that can simultaneously determine all binding parameters in a single experiment: affinity constant (KD), reaction stoichiometry (n), and thermodynamic parameters such as enthalpy (∆H) and entropy (ΔS) to elucidate the mechanisms underlying molecular interactions. Requiring no modification of binding partners, either with fluorescent tags or through immobilization, ITC measures the affinity of binding partners in their native states.
SPR-CGI
At Alfatestlab we use the Creoptix® WAVE system based on SPR-GCI or SPR-Grating-Coupled Interferometry technology that combines very high robustness and high sensitivity for the study of interaction kinetics, label-free and in real-time. The advantage of this technology over classic SPR is that it makes it possible to generate interaction and kinetic data on even the most difficult samples, guaranteeing high analytical and experimental flexibility.